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Plant and Cell Physiology, 1996, Vol. 37, No. 2 201-205
© 1996

Structure and Expression of Two Seed-Specific cDNA Clones Encoding Stearoyl-Acyl Carrier Protein Desaturase from Sesame, Sesamum indicum L.

Yasushi Yukawa1,4, Fumio Takaiwa2, Kazuaki Shoji3, Kyojiro Masuda1 and Kyoji Yamada1,5

1Department of Biology, Faculty of Science, Toyama University Gofuku, Toyama, 930 Japan
2National Institute of Agrobiological Resources Kan-nondai, Tsukuba, 305 Japan
3Biotechnology Section, Toyama Agricultural Research Center Yoshioka, Toyama, 939 Japan

5To whom correspondence should be addressed.

We have isolated two cDNA clones (CDES01 and 04) encoding stearoyl-acyl carrier protein desaturase (SACPD; EC 1.14.99.6 [EC] ) from immature sesame seeds, and have analyzed accumulation levels of the corresponding mRNAs at different stages and organs in sesame. Clone CDES01 contains an open reading frame coding for a 396-amino acid protein of 45 kDa. CDES04 encodes a partial sequence of 141-amino acids. Deduced amino acid sequences of both clones exhibit a high identity to those of other plant SACPD cDNAs. Northern blots probed with CDES01 and CDES04 indicate that both messages accumulate in a seed-specific manner with a peak at 21 days after anthesis. However, expression patterns also indicate that regulation between CDES01 and CDES04 are slightly different. The CDES01 message accumulates at a low level in young leaves in addition to seeds, whereas accumulation of the RNA transcript corresponding to CDES04 is restricted to seeds. This observation implies the presence of at least two isozymes of SACPD having overlapping but slightly distinct functions in sesame.

4Present address: Center for Gene Research, Nagoya University, Nagoya, 464-01 Japan

(Received October 17, 1995; Accepted March 5, 1996)
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