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Plant and Cell Physiology, 1996, Vol. 37, No. 2 141-146
© 1996

NADH-Linked Electron Transfer Induces Cd2+ Movement in Corn Root Plasma Membrane Vesicles

Shu-I Tu, Deidre Patterson, Siyuan Shen, David Brauer and An-Fei Hsu

USDA/ARS/NAA, Eastern Regional Research Center 600 E. Mermaid Lane, Wyndmoor, PA 19118, U.S.A.

The rate of electron transfer from NADH to ferricyanide associated with corn root plasma membrane vesicles was not significantly affected by the presence of Ca2+, Cd2+, Zn2+, or Co2+. However, unlike Ca2+, other tested divalent cations induced an electron transfer-dependent increase in light scattering by the vesicles. The efficacy in inducing the light scattering increase followed the order of Cd2+>Zn2+>Co2+. The extent of the light scattering change increased as the concentration of added Cd2+ increased. Using phase contrast microscopy, the increase in light scattering was related to an expansion of the vesicles. Accompanying the volume increase, a decrease of Cd2+ concentration in the external space was observed by the use of Arsenazo III, a metallochromophore specific for divalent cations. Under a specified electron transfer condition, a similar percentage of decrease over a wide range of initial Cd2+ concentration was detected. The presence of A23187 [GenBank] decreased the rate and extent of light scattering increase and its associated Cd2+ concentration change. However, A23187 [GenBank] had no effect on scattering changes already established by the electron transfer and Cd2+. These results suggest that the NADH linked electron transfer reaction may induce a Cd2+ uptake by the vesicles of corn root plasma membrane with inside-out orientation.

(Received September 25, 1995; Accepted December 19, 1995)
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