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Plant and Cell Physiology, 1995, Vol. 36, No. 8 1621-1627
© 1995

The 5' Flanking Sequences of Two S Alleles in Lycopersicon peruvianum Are Highly Heterologous but Contain Short Blocks of Homologous Sequences

Kyung Chung, II1, Sang Yeb Lee1, Toru Ito2, Hiroshi Tanaka2, Hong Gil Nam1,3 and Masamichi Takagi2,4

1 Department of Life Science, Pohang University of Science and Technology Pohang, Kyungbuk 790-784, South Korea
2 Department of Biotechnology, The University of Tokyo Bunkyo-ku, Tokyo, 113 Japan
3 Plant Molecular Biology and Biotechnology Research Center Jinju, Kyungnam 660-701, South Korea

4Corresponding author.

To characterize the molecular structure of the S alleles in Lycopersicon peruvianum, we isolated two genomic fragments that corresponded to cDNAs for S11 and S12 RNases from a genomic library of a plant with the S11S12 genotype. The coding regions and the flanking regions in both fragments were sequenced. A single short intron was found in each gene for RNase, and it was located in the previously identified HV2 region [Chung et al. (1994) Plant Mol. Biol. 26: 757]. The site of initiation of transcription of the gene for S11 RNase was determined by a primer extension analysis and a putative TATA box was identified. Three short sequences that were highly homologous in the two S alleles were found within 360 bp upstream of the ATG initiation codon. It is suggested that these sequences might be involved in the regulation of transcription required for the appropriate-level or tissue-specific expression of the S genes in L. peruvianum.

(Received July 11, 1995; Accepted October 2, 1995)
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