Plant and Cell Physiology, 1995, Vol. 36, No. 8 1555-1562
© 1995
The Sequence and Expression of the 
-VPE Gene, One Member of a Family of Three Genes for Vacuolar Processing Enzymes in Arabidopsis thaliana
1 Department of Cell Biology, National Institute for Basic Biology Okazaki, 444 Japan
2 Department of Molecular Biomechanics, School of Life Science, The Graduate University for Advanced Studies Okazaki, 444 Japan
3To whom correspondence should be addressed.
Vacuolar processing enzymes (VPEs) are responsible for the maturation of seed proteins. Southern blot analysis showed that a family of genes for VPEs in Arabidopsis thaliana was composed of three genes, for 
-VPE, rß-VPE and 
-VPE, respectively. The gene for -VPE was isolated from a genomic library. It was composed of nine exons and eight introns. The positions of the introns were fully conserved among the three genes, with the exception that the -VPE gene was missing the fifth intron found in the rß-VPE and -VPE genes. The predicted -VPE protein was 80% and 57% identical in terms of amino acid sequence to the -VPE protein and rß-VPE protein, respectively. Northern blot analysis demonstrated that the -VPE gene was expressed predominantly in the stems, with a lower level of expression in rosette and cauline leaves. However, the expression was not detected in roots, flowers plus buds, or green siliques, in contrast to the high-level expression of the rß-VPE gene in the flowers plus buds. Thus, -VPE seems to be an isoform that is specific to vegetative organs. Members of the VPE family can be separated into two subfamilies, one that is specific to seeds and another that is specific to vegetative organs, such as leaves and stems. The members of the seed subfamily might function in the protein-storage vacuoles of seeds, while those of the vegetative subfamily might function in the lytic vacuoles of non-storage organs.
(Received May 17, 1995; Accepted September 18, 1995)
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