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Plant and Cell Physiology, 1995, Vol. 36, No. 8 1531-1539
© 1995

Molecular Cloning and Analysis of the cDNA for an Auxin-Regulated Calmodulin Gene

Hitoshi Okamoto1, Yoshiyuki Tanaka2 and Shingo Sakai1,

1 Institute of Biological Sciences, University of Tsukuba Tsukuba, Ibaraki, 305 Japan
2 Chemical Tolerance, National Institute of Agrobiological Resources Tsukuba, Ibaraki, 305 Japan

S. Sakai; FAX 81 (291) 53 4579.

An auxin-regulated calmodulin cDNA (arCaM) were isolated by differential screening from a mung bean (Vigna radiata) cDNA library. The expression of the arCaM transcript in the etiolated mung bean hypocotyl was examined by RNA gel blot analysis. The arCaM transcript was induced depending on indole-3-acetic acid (IAA) concentrations. An increase in level of the arCaM transcript upon treatment of hypocotyl segments with 10 µM IAA was detected after 1 h and a maximum level was detected at 2 h. Induction of the arCaM transcript occurred upon treatment with 10 µM 2,4-dichlorophenoxyacetic acid (2,4-D) or naphthalene-1-acetic acid (NAA) as well as with IAA, while treatment with 10 µM p-chlorophenoxyisobutyric acid (PCIB) as an anti-auxin, prevented the induction. Ethylene did not have any effect. Other stress conditions, such as exposure to salt stress, heavy metal ions and heat shock, also had no effect on the induction. The levels of the arCaM transcript in leaves of light-grown mung bean plants treated with IAA showed steady but small increases with time.

(Received May 26, 1995; Accepted September 11, 1995)
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