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Plant and Cell Physiology, 1995, Vol. 36, No. 7 1341-1348
© 1995

A Recombinant Rice 16.9-kDa Heat Shock Protein Can Provide Thermoprotection in Vitro

Ching-Hui Yeh, Kai-Wun Yeh, Shu-Hsing Wu, Pi-Fang Linda Chang, Yih-Ming Chen and Chu-Yung Lin1

Department of Botany, National Taiwan University Taipei, Taiwan, R.O.C.

1To whom correspondence should be addressed.

It is difficult to obtain large amounts of purified low-molecular-mass heat shock proteins (LMM HSPs), which are unique to plants, for biochemical and physiological studies. Therefore, an attempt was made to produce such a HSP by applying recombinant DNA technology. We fused the cDNA for a rice class I 16.9-kDa HSP, pTSl, to the gene for glutathione S-transferase (GST) of Schistosoma japonicum and we obtained large amounts of the fusion protein from transformed Escherichia coli cells. In addition, we found that the 16.9-kDa HSP obtained by cleavage of the recombinant protein could also form a protein complex of {small tilde}310 kDa under non-denaturing conditions as can the small, native, class I HSPs from heat-shocked rice seedlings. An assay in vitro to examine the thermoprotection of rice soluble proteins from heat denaturation revealed the strong stabilizing effect of the recombinant HSP.

(Received March 13, 1995; Accepted August 17, 1995)
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