Plant and Cell Physiology, 1995, Vol. 36, No. 7 1331-1339
© 1995
Effects of Sulfate Concentrations on the Expression of a Soybean Seed Storage Protein Gene and its Reversibility in Transgenic Arabidopsis thaliana
1 Department of Applied Biological Chemistry, Division of Agriculture and Agricultural Life Sciences, The University of Tokyo Yayoi, Bunkyo-ku, Tokyo, 113 Japan
2 Department of Applied Bioscience, Faculty of Agriculture, Hokkaido University Sapporo, 060 Japan
4To whom correspondence should be addressed.
Transgenic expression of genes encoding the a' and p subunits of rß-conglycinin, one of the major seed storage proteins of soybean (Glycine max [L.] Merr.), was analyzed in Arabidopsis thaliana (L.) Heynh. under conditions of sulfate deficiency. Temporal patterns of expression of both the intact rß subunit gene and the rß subunit gene promoter fused to the rß-glucuronidase (GUS) gene are similar in soil-less cultures using rockwool, suggesting that the response to sulfate deficiency is regulated mainly at the level of transcription. In hydroponic cultures with various concentrations of sulfate, expression of both the intact rß subunit gene and the rß subunit gene promoter-GUS fusion gene were negatively correlated to increased sulfate concentrations in the culture medium. Transfer of transgenic A. thaliana plants carrying the rß subunit gene promoter-GUS fusion from sulfate-deficient to sulfate-sufficient control medium caused GUS activity in developing siliques to be repressed within two days. A reverse shift, where the plants were transferred from the control to sulfate-deficient medium, caused GUS activity to become higher than that in seeds of the control plants within two days. These results indicate that the expression of the rß subunit gene promoter responds rapidly to changes of sulfate availability.
3Recipient of a Fellowship for Japanese Junior Scientists from the Japan Society for the Promotion of Science.
(Received June 8, 1995; Accepted August 16, 1995)
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