Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Yazaki, K.
Right arrow Articles by Tabata, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yazaki, K.
Right arrow Articles by Tabata, M.
Agricola
Right arrow Articles by Yazaki, K.
Right arrow Articles by Tabata, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 1995, Vol. 36, No. 7 1319-1329
© 1995

Isolation and Characterization of Two cDNAs Encoding 4-Coumarate:CoA Ligase in Lithospermum Cell Cultures

Kazufumi Yazaki1, Atsushi Ogawa and Mamoru Tabata

Faculty of Pharmaceutical Sciences, Kyoto University Yoshida, Kyoto, 606-01 Japan

1Corresponding author.

Two near full-length cDNAs (LE4CL-1, LE4CL-2), which encode 4-coumarate:CoA ligase (4CL), were cloned from a library of Lithospermum erythrorhizon cell suspension cultures by the use of heterologous probe of potato 4CL. These cDNAs are 2.1 kb and 2.2 kb in length, respectively. LE4CL-1 encodes 636 amino acids, whose homologies to the 4CL protein sequences known to potato, parsley, pine and rice, were found to be 68%, 66%, 56% and 50% (identities on amino acid level), respectively, whereas those of the predicted translation product of LE4CL-2 (594 amino acids) to the above 4CL proteins were 49{small tilde}54%. The similarity of the deduced amino acid sequences between the two 4CLs from Lithospermum cell cultures was 49% in identity. Northern analyses showed that the mRNA levels of both LE4CL-1 and LE4CL-2 were much higher under illumination than in the dark, as reported for the 4CL genes of such plants as parsley. In comparison of mRNA levels of LE4CL-1 and LE4CL-2, the former was demonstrated to be generally higher than the latter by means of an application of RT-PCR. The genomic southern blot experiments suggested that there are probably three copies of LE4CL-1 in the Lithospermum genome DNA, whereas only one copy was detected for LE4CL-2.

(Received May 26, 1995; Accepted August 16, 1995)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant Cell PhysiolHome page
Y. Yamamura, F. P. Sahin, A. Nagatsu, and H. Mizukami
Molecular Cloning and Characterization of a cDNA Encoding a Novel Apoplastic Protein Preferentially Expressed in a Shikonin-Producing Callus Strain of Lithospermum erythrorhizon
Plant Cell Physiol., April 15, 2003; 44(4): 437 - 446.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Yazaki, M. Kunihisa, T. Fujisaki, and F. Sato
Geranyl Diphosphate:4-Hydroxybenzoate Geranyltransferase from Lithospermum erythrorhizon. CLONING AND CHARACTERIZATION OF A KEY ENZYME IN SHIKONIN BIOSYNTHESIS
J. Biol. Chem., February 15, 2002; 277(8): 6240 - 6246.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.