Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Iwano, M.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Iwano, M.
Agricola
Right arrow Articles by Iwano, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 1995, Vol. 36, No. 7 1297-1301
© 1995

Selective Effect of Salt Stress on the Activity of Two ATPases in the Cell Membrane of Nostoc muscorum

Megumi Iwano

ATPase activity in the cell membrane of a salt-stressed cyanobacterium, Nostoc muscorum M-14, was examined cytochemically by three different staining protocols. Application of Hulstaert's method resulted in distinct precipitation of the reaction products of ATPase inside the cell membrane exclusively. No reaction products were formed when ATP was replaced by GTP or when dicyclohexylcarbodiimide or N-ethylmaleimide was present in the reaction mixture. By contrast, low levels were detectable after the reaction in the presence of ouabain. Bafilomycin did not affect the formation of products. Mayahara's method, which is considered to demonstrate the reaction of Na+,K+-ATPase activity, revealed the presence of a ouabain-sensitive Na+,K+-ATPase in the cell membrane, while Wachstein-Meisel's method revealed the presence of an ATPase activity that was resistant to ouabain. It appears, therefore, that cell membranes of Nostoc muscorum contain both ouabain-sensitive ATPase and ouabain-insensitive ATPase. Comparison of the staining profiles of salt-stressed cells with those of control cells suggested that a high-salt environment activates the ouabain-sensitive Na+,K+-ATPase, which seems likely to be involved in the efflux of Na+ ions.

(Received February 7, 1995; Accepted August 9, 1995)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
P. V. Minorsky

Plant Physiology, September 1, 2002; 130(1): 4 - 5.
[Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.