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Plant and Cell Physiology, 1995, Vol. 36, No. 7 1291-1296
© 1995

Characterization of Sulfate Transport in the Green Alga Chlorella ellipsoidea

Yusuke Matsuda1 and Brian Colman

Department of Biology, York University 4700 Keele Street, North York, Ontario, Canada, M3J 1P3

1To whom correspondence should be addressed.

A rapid induction of sulfate transport was observed in the green alga Chlorella ellipsoidea during sulfur-limited growth. Both affinity and Vmax increased about five-fold within 6 h of transferring cells from Bold's basal medium with 350 µM MgSO4 to sulfur-deficient Bold's medium. High affinity sulfate transport was induced within 15 min and reached maximum rate within 3 h of transferring cells to sulfur-deficient condition, indicating that a new, high-affinity-sulfate transport system is induced by sulfur starvation in C. ellipsoidea. Eadie-Hofstee plots of initial rates of sulfate uptake indicated that the K1/2 of sulfur-starved cells was about 17 µM. Both sulfur-starved and unstarved cells grown in air had a Vmax of 1.5 times higher than that of high-CO2 grown cells. Sulfate transport was completely inhibited by 30 µM CCCP or 800µM KCN both in the light and the dark but transport in the light was not inhibited by 20 µM DCMU. Treatment with 50 µM or 500 µM vanadate caused 50% inhibition of uptake. The rate of sulfate uptake in the dark was twice that in the light and was stimulated by low pH. These results suggest that the sulfate transport system in C. ellipsoidea is operated by proton symport across the plasmamembrane which is partially mediated by P-type ATPase and that these systems depend exclusively on energy derived from oxidative phosphorylation in the mitochondria.

(Received June 28, 1995; Accepted August 8, 1995)
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