Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Suzuki, G.
Right arrow Articles by Hinata, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Suzuki, G.
Right arrow Articles by Hinata, K.
Agricola
Right arrow Articles by Suzuki, G.
Right arrow Articles by Hinata, K.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 1995, Vol. 36, No. 7 1273-1280
© 1995

Molecular Cloning of Members of the S-Multigene Family in Self-Incompatible Brassica campestris L.

Go Suzuki1, Masao Watanabe1, Kinya Toriyama1, Akira Isogai2 and Kokichi Hinata1

1 Faculty of Agriculture, Tohoku University Sendai, 981 Japan
2 Graduate School of Biological Sciences, Nara Advanced Institute of Science and Technology Ikoma, 630-01 Japan

We isolated 12 groups of genomic clones that contained SLG-homologous regions from a genomic library constructed from an S9 homozygote of self-incompatible Brassica campestris. Both SLG9 and SRK9 genomic clones, which are located within the self-incompatibility (S) locus, were included in these groups. The promoter regions of SLG9 and SRK9 were completely identical for at least 200 bp upstream from their respective initiation codons (ATG). The five sequence elements (boxes I to V) that are conserved in the promoters of SLG and SRK genes were also found in the SLG9 and SRK9 clones. However, one conserved element (box III) unexpectedly lacked 7 of 11 bp, although box III has been considered necessary for expression in pistil. The other ten groups of genomic clones were classified into six SRK-like groups and four SLG-like groups. These results indicate that SLG, SRK, SLG-like, and SRK-like genes form a large S-multigene family in B. campestris.

(Received April 26, 1995; Accepted August 4, 1995)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant CellHome page
H. Shimosato, N. Yokota, H. Shiba, M. Iwano, T. Entani, F.-S. Che, M. Watanabe, A. Isogai, and S. Takayama
Characterization of the SP11/SCR High-Affinity Binding Site Involved in Self/Nonself Recognition in Brassica Self-Incompatibility
PLANT CELL, January 1, 2007; 19(1): 107 - 117.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. Takayama and A. Isogai
Molecular mechanism of self-recognition in Brassica self-incompatibility
J. Exp. Bot., January 1, 2003; 54(380): 149 - 156.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
M. Watanabe, K. Hatakeyama, Y. Takada, and K. Hinata
Molecular Aspects of Self-Incompatibility in Brassica Species
Plant Cell Physiol., June 1, 2001; 42(6): 560 - 565.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
G. Suzuki, N. Kai, T. Hirose, K. Fukui, T. Nishio, S. Takayama, A. Isogai, M. Watanabe, and K. Hinata
Genomic Organization of the S Locus: Identification and Characterization of Genes in SLG/SRK Region of S9 Haplotype of Brassica campestris (syn. rapa)
Genetics, September 1, 1999; 153(1): 391 - 400.
[Abstract] [Full Text]

Home page
 GeneticsHome page
K. Hatakeyama, T. Takasaki, M. Watanabe, and K. Hinata
Molecular Characterization of S Locus Genes, SLG and SRK, in a Pollen-Recessive Self-Incompatibility Haplotype of Brassica rapa L
Genetics, July 1, 1998; 149(3): 1587 - 1597.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. Ansaldi, A. Chaboud, and C. Dumas
Multiple S Gene Family Members Including Natural Antisense Transcripts Are Differentially Expressed during Development of Maize Flowers
J. Biol. Chem., July 28, 2000; 275(31): 24146 - 24155.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Takayama, H. Shiba, M. Iwano, K. Asano, M. Hara, F.-S. Che, M. Watanabe, K. Hinata, and A. Isogai
Isolation and characterization of pollen coat proteins of Brassica campestris that interact with S locus-related glycoprotein 1 involved in pollen-stigma adhesion
PNAS, March 28, 2000; 97(7): 3765 - 3770.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.