Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Messiaen, J.
Right arrow Articles by Van Cutsem, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Messiaen, J.
Right arrow Articles by Van Cutsem, P.
Agricola
Right arrow Articles by Messiaen, J.
Right arrow Articles by Van Cutsem, P.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 1995, Vol. 36, No. 7 1213-1220
© 1995

Callose Synthesis in Spirostanol Treated Carrot Cells is Not Triggered by Cytosolic Calcium, Cytosolic pH or Membrane Potential Changes

Johan Messiaen, Frédéric Nérinckx and Pierre Van Cutsem

Facultés Universitaires Notre-Dame de la Paix, Laboratoire de Biotechnologie théorique rue de Bruxelles 61, B-5000 Namur, Belgium

Carrot (Daucus carota L.) cell suspensions were treated with a spirostanol saponin from Yucca. This saponin is an elicitor of callose synthesis. Irrespectively of the mode of action of spirostanol on the callose synthase activity itself, the spirostanol-induced callose synthesis in carrot is not preceded by changes in membrane potential, cytosolic free calcium or cytosolic pH. The inability of modulators of cytosolic free calcium content (verapamil, nifedipine and Br-A23187), EGTA and a proton pump inhibitor (vanadate) to inhibit or induce callose formation is consistent with a calcium- and pH-independent mechanism for callose deposition.

(Received March 20, 1995; Accepted July 18, 1995)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.