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Plant and Cell Physiology, 1995, Vol. 36, No. 5 919-924
© 1995

Short Communication

Isolation of cDNA for a Plasma Membrane H+-ATPase from Guard Cells of Vicia faba L.

Nobuyoshi Nakajima, Hikaru Saji, Mitsuko Aono and Noriaki Kondo

National Institute for Environmental Studies Tsukuba, 305 Japan

cDNA encoding the plasma membrane H+-ATPase of guard cells of Vicia faba L. was isolated. The clone encoded a 105-kDa polypeptide (956 amino acids) that was 79–85% identical in terms of amino acid sequence to other plant H+-ATPases. High levels of mRNA explain the high H+-ATPase activity of these plasma membranes.

(Received December 24, 1994; Accepted April 12, 1995)
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