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Plant and Cell Physiology, 1995, Vol. 36, No. 5 857-864
© 1995

Photoactive Photosystem I Particles with a Molar Ratio of Chlorophyll a to P700 of 9

Isamu Ikegami1, Shigeru Itoh2 and Masayo Iwaki2

1Faculty of Pharmaceutical Sciences, Teikyo University Sagamiko, Kanagawa, 199-01 Japan
2National Institute for Basic Biology Okazaki, 444 Japan

Lyophilized photosystem I particles from spinach were treated with diethyl ether that contained various organic solvents with different dielectric constants. More pigments were extracted as the dielectric constant of the solvent added to ether increased. The reaction-center chlorophylldimer, P700, was more resistant to extraction than the rest of the chlorophyll. Particles that contained only 6 chlorophylls in addition to P700 and the primary electron acceptor (A0), in a single reaction-center unit, were prepared by extraction with a mixture of ether and acetaldehyde. A distinct shoulder at 695 nm due to P700 or at 686 nm due to P700+ was observed in the absorption spectra of the reduced or oxidized particles, respectively, even at room temperature. No secondary acceptor phylloquinone remained in the particles. Stable charge separation was restored upon the addition of 2-amino-anthraquinone, even though the particles had the lowest molar ratio of chlorophyll to P700 of any reported particles.

(Received February 20, 1995; Accepted May 8, 1995)
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I. Ikegami, S. Itoh, and M. Iwaki
Selective Extraction of Antenna Chlorophylls, Carotenoids and Quinones from Photosystem I Reaction Center
Plant Cell Physiol., October 1, 2000; 41(10): 1085 - 1095.
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