Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Sakakibara, H.
Right arrow Articles by Sugiyama, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sakakibara, H.
Right arrow Articles by Sugiyama, T.
Agricola
Right arrow Articles by Sakakibara, H.
Right arrow Articles by Sugiyama, T.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 1995, Vol. 36, No. 5 789-797
© 1995

Isolation and Characterization of a cDNA That Encodes Maize Glutamate Dehydrogenase

Hitoshi Sakakibara1, Kazutoshi Fujii2 and Tatsuo Sugiyama

Department of Applied Biological Sciences, School of Agricultural Sciences, Nagoya University Nagoya, 464-01 Japan

1To whom correspondence should be addressed.

A full-length cDNA clone, pMGDHl, encoding maize NADH-glutamate dehydrogenase (NADH-GDH) was isolated from a maize root cDNA library. The identity of the cDNA was established by the coincidence of the structure of the purified protein with that inferred from the nucleotide sequence of the cDNA. pMGDHl had a cDNA insert of 1,638 bp and the open reading frame encoded 411 amino acid residues. The deduced amino acid sequence was similar to putative partial sequences of GDHs from higher plants and to the sequences of GDHs from organisms as diverse as mammals and bacteria. The NH2-terminal sequence deduced from the open reading frame had a typical structure that is associated with the import of proteins into the mitochondrial matrix. The cDNA hybridized to an RNA of about 1.6 kb. This transcript was more abundant in roots than in leaves and was localized in the bundle sheath cells in leaf tissues. Analysis of genomic DNA by Southern hybridization suggested the existence of gene(s) for another NADHGDH subunit(s).

2Present address: Biochemical Research Laboratories, Ezaki Glico Co., Ltd.4-6-5 Utajima, Nishiyodogawa-ku, Osaka, 555 Japan

(Received January 30, 1995; Accepted April 11, 1995)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant Cell PhysiolHome page
T. Abiko, M. Obara, A. Ushioda, T. Hayakawa, M. Hodges, and T. Yamaya
Localization of NAD-Isocitrate Dehydrogenase and Glutamate Dehydrogenase in Rice Roots: Candidates for Providing Carbon Skeletons to NADH-Glutamate Synthase
Plant Cell Physiol., October 1, 2005; 46(10): 1724 - 1734.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
A. Gallais and B. Hirel
An approach to the genetics of nitrogen use efficiency in maize
J. Exp. Bot., February 1, 2004; 55(396): 295 - 306.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
B. Hirel, P. Bertin, I. Quilleré, W. Bourdoncle, C. Attagnant, C. Dellay, A. Gouy, S. Cadiou, C. Retailliau, M. Falque, et al.
Towards a Better Understanding of the Genetic and Physiological Basis for Nitrogen Use Efficiency in Maize
Plant Physiology, March 1, 2001; 125(3): 1258 - 1270.
[Abstract] [Full Text]

Home page
 J Exp BotHome page
S. Aubert, R. Bligny, R. Douce, E. Gout, R.G. Ratcliffe, and J.K.M. Roberts
Contribution of glutamate dehydrogenase to mitochondrial glutamate metabolism studied by 13C and 31P nuclear magnetic resonance
J. Exp. Bot., January 1, 2001; 52(354): 37 - 45.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
Y. Onda, T. Matsumura, Y. Kimata-Ariga, H. Sakakibara, T. Sugiyama, and T. Hase
Differential Interaction of Maize Root Ferredoxin:NADP+ Oxidoreductase with Photosynthetic and Non-Photosynthetic Ferredoxin Isoproteins
Plant Physiology, July 1, 2000; 123(3): 1037 - 1046.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
T. Matsumura, Y. Kimata-Ariga, H. Sakakibara, T. Sugiyama, H. Murata, T. Takao, Y. Shimonishi, and T. Hase
Complementary DNA Cloning and Characterization of Ferredoxin Localized in Bundle-Sheath Cells of Maize Leaves
Plant Physiology, February 1, 1999; 119(2): 481 - 488.
[Abstract] [Full Text]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.