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Plant and Cell Physiology, 1995, Vol. 36, No. 1 79-84
© 1995

Purification and Characterization of a Novel Sex Pheromone that Induces the Release of Another Sex Pheromone during Sexual Reproduction of the Heterothallic Closterium peracerosum-strigosum-littorale Complex

Takahiro Nojiri, Tadashi Fujii and Hiroyuki Sekimoto1

Institute of Biological Sciences, University of Tsukuba Tsukuba, Ibaraki, 305 Japan

A sex pheromone, PR-IP Inducer, which is released from mating-type minus cells of Closterium, was purified by monitoring its biological effect on the induction of the release of protoplast-release-inducing protein (PR-IP) from mating-type plus cells. The purified PR-IP Inducer had an apparent molecular mass of 22 kDa and of 18.7 kDa as determined by SDS-PAGE and mass-spectrometric analysis, respectively. Staining with periodic acid-Schiff reagent indicated that PR-IP Inducer included a glycan chain. From the analysis of a dose-response curve, it seemed that PR-IP Inducer was able to exert its activity over quite wide range of concentrations (1 x 10–10–3 x 10–7 M). It appears that PR-IP Inducer is a novel glycoproteinaceous pheromone, as is PR-IP, and that it exerts its effect at the earliest stages of the sexual reproduction of the Closterium peracerosum-strigosum-littorale complex.

1Recipient of a Fellowship for Japanese Junior Scientists from the Japan Society for the Promotion of Science.

(Received August 5, 1994; Accepted November 1, 1994)
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