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Plant and Cell Physiology, 1995, Vol. 36, No. 1 53-58
© 1995

Furosemide: a Specific Inhibitor of Pi Transport across the Plasma Membrane of Plant Cells

Kiyoshi Okihara1, Tetsuro Mimura2,3, Seiichiro Kiyota1 and Katsuhiro Sakano1

1 Department of Applied Physiology, National Institute of Agrobiological Resources Tsukuba, 305 Japan
2 Department of Life Science, Himeji Institute of Technology Harima Science Park City, 678-22 Japan

A search was made for inhibitors of Pi uptake that act directly on the Pi transporter in the plasma membranes of Catharanthus roseus cells to inhibit Pi uptake without inhibition of proton pumping. Using standard electrodes, we monitored changes in pH and in the concentration of K+ ions, as well as the rate of Pi uptake, when an inhibitor to be tested was applied to the cells in unbuffered medium. A9C (28 µM), a blocker of anion channels, inhibited Pi uptake but it also inhibited the proton pump. However, a structurally similar inhibitor, furosemide, inhibited Pi uptake without inhibiting proton pumping.

It is suggested that the carboxylic group of these inhibitors interacts with the Pi-binding site (probably an amino group) of the Pi transporter in the plasma membrane and that the hydrophobic structure of these inhibitors facilitates their accumulation in the plasma membrane.

3Present address: Department of Biology, Hitotsubashi University, 2-1 Naka, Kunitachi, Tokyo, 186 Japan

(Received May 26, 1994; Accepted October 24, 1994)
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