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Plant and Cell Physiology, 1995, Vol. 36, No. 1 37-44
© 1995

Tissue-Dependent Expression of the Rice wx+ Gene Promoter in Transgenic Rice and Petunia

Hiro-Yuki Hirano1, Noriko Tabayashi2, Takeshi Matsumura2, Masatoshi Tanida2, Yoshibumi Komeda3,4 and Yoshio Sano1

1 National Institute of Genetics Yata, Mishima, 411 Japan
2 Hokkaido Green-Bio Institute Naganuma, Hokkaido, 069-13 Japan
3 Molecular Genetics Research Laboratory, University of Tokyo 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113 Japan

The waxy (wx) locus, which controls the amylose synthesis, is known to be expressed specifically in the endosperm and pollen. To study the tissue-specific regulation of the wx+ gene, we introduced a fusion gene that consisted of the upstream sequence of the wx+ gene and the gene for rß-glucuronidase (GUS) into cells of rice (Oryza sativa L.) and petunia (Petunia hybrida L.). GUS activity was examined in the regenerated transgenic rice and petunia plants. In transgenic rice, the upstream sequence of the wx+ gene was sufficient to direct the tissue-specific expression of GUS in the endosperm and pollen, and the control of expression was quantitative. By contrast, in transgenic petunia, the same fusion gene was expressed in pollen but not in the endosperm. These results suggest that the putative cis-acting elements that direct pollen-specific expression are common to or similar in both monocotyledonous and dicotyledonous plants, whereas ciy-elements responsible for the endosperm-specific expression of the rice wx+ gene do not function in petunia, in which development of the endosperm differs from that in rice.

4Present address: Division of Biological Sciences, Graduate School of Science, Hokkaido University, Kita-ku, Sapporo, 060 Japan

(Received April 27, 1994; Accepted October 11, 1994)
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