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Plant and Cell Physiology, 1994, Vol. 35, No. 8 1121-1125
© 1994

Inhibitory Effect of Thidiazuron on the Activity of Cytokinin Oxidase Isolated from Soybean Callus

Peter D. Hare and Johannes Van Staden1

NU Research Unit for Plant Growth and Development, Department of Botany, University of Natal Pietermaritzburg 3200, Republic of South Africa

1Corresponding author.

Cytokinin oxidase activity was demonstrated in a 40–80% ammonium sulphate cut of total proteins from soybean cotyledonary callus. The cytokinin-active di-substituted urea, thidiazuron (TDZ) inhibited cytokinin oxidase activity by a mixed, predominantly uncompetitive mechanism. This result is discussed as one of several possible modes of action of phenylurea-type cytokinins, which represent a problem in cytokinin structure-activity relationships.

(Received March 11, 1994; Accepted July 13, 1994)
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