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Plant and Cell Physiology, 1994, Vol. 35, No. 6 943-950
© 1994

Light-Induced Changes in Cytosolic pH in Leaf Cells of Egeria densa: Measurements with pH-Sensitive Microelectrodes

Yoshiji Okazaki1, Masashi Tazawa2 and Naohiko Iwasaki1

1Department of Biology, Osaka Medical College Sawaragicho 2-41, Takatsuki, Osaka, 569 Japan
2Department of Applied Physics and Chemistry, Fukui Institute of Technology Gakuen 3-6-1, Fukui, 910 Japan

Light-induced changes of cytosolic pH (pHc) and the plasmalemma potential (Em) in dark-adapted leaf cells of the aquatic plant, Egeria densa were measured simultaneously with double-barreled pH-sensitive microelectrodes. Upon illumination, pHc increased transiently and then decreased to a level that was lower than the original value, while the plasmalemma was greatly hyperpolarized after an initial small depolarization. DCMU inhibited the light-induced changes in both pHc and Em. DCMU acted without directly inhibiting the electrogenic proton pump in the plasmalemma since a decrease in pHc caused by treatment with butyrate (H+-loading) hyperpolarized the plasmalemma in DCMU-pretreated cells. N.N-Dicyclohexylcarbodiimide (DCCD) also inhibited the light-induced changes in both pHc and Em. This result may be explained by direct inhibition of the proton pump in the plasmalemma by DCCD since the decrease in pHc caused by butyrate did not induce membrane hyperpolarization in DCCD-treated leaf cells. Fusicoccin induced membrane hyperpolarization and slight acidification of the cytosol. DCCD inhibited the fusicoccin-induced changes in both pHc and Em. The mechanism of the light-induced changes in pHc is discussed in relation to activities of the proton pump in the plasmalemma and photosynthesis.

(Received January 10, 1994; Accepted June 9, 1994)
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