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Plant and Cell Physiology, 1994, Vol. 35, No. 6 927-934
© 1994

Differential Regulation of Soybean Seed Storage Protein Gene Promoter-GUS Fusions by Exogenously Applied Methionine in Transgenic Arabidopsis thaliana

Masami Yokota Hirai1, Toru Fujiwara1, Koji Goto2, Yoshibumi Komeda2,3, Mitsuo Chino1 and Satoshi Naito2,4

1Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo Yayoi, Bunkyo-ku, Tokyo, 113 Japan
2Molecular Genetics Research Laboratory, The University of Tokyo Hongo, Bunkyo-ku, Tokyo, 113 Japan

Expression of the gene encoding the ß subunit of ß-conglycinin, the 7S seed storage protein of soybean (Glycine max [L.] Merr.), has been shown to be down-regulated by exogenously applied L-methionine in in vitro soybean cotyledon cultures, whereas expression of the gene encoding the a' subunit is not affected. We constructed transgenic Arabidopsis thaliana(L.) Heynh. carrying the ß-glucuronidase (GUS) reporter gene under the control of the promoter regions from either the a' or ß subunit gene. An in vitro culture system of immature siliques of A. thaliana was developed for exogenous application of L-methionine to immature seeds. Expression of the fi subunit gene promoter-GUS fusion was down-regulated by the application of L-methionine, and this response was reversible. As an alternative way to apply L-methionine, A. thaliana plants were grown on rockwool and irrigated with L-methionine-supplemented medium. GUS activity driven by the ß subunit gene promoter was repressed by L-methionine, and the degree of repression depended on the concentration of L-methionine in the medium. On the other hand, the a' subunit gene promoter-GUS fusion did not show such a response. These results indicate that A. thaliana has a system to differentially regulate the expression of the ß-conglycinin genes by L-methionine at the level of transcription. This work establishes a system to genetically analyze plants' responses to nutritional stimuli.

3Present address: Division of Biological Sciences, Graduate School of Science, Hokkaido University, Kita-ku, Sapporo, 060 Japan

4Present address: Department of Applied Bioscience, Faculty of Agriculture, Hokkaido University, Kita-ku, Sapporo, 060 Japan

(Received April 21, 1994; Accepted June 6, 1994)
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