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Plant and Cell Physiology, 1994, Vol. 35, No. 6 893-899
© 1994

Effects of the Degree of Polymerization on the Binding of Xyloglucans to Cellulose

Takahisa Hayashi1,1, Takumi Takeda1, Kozo Ogawa2 and Yasushi Mitsuishi3

1Wood Research Institute, Kyoto University Gokasho, Uji, Kyoto, 611 Japan
2Research Institute for Advanced Science and Technology, University of Osaka Prefecture 1-2 Gakuen-cho, Sakai, Osaka, 593 Japan
3National Institute of Bioscience and Human Technology AIST, 1-1 Higashi, Tsukuba, Ibaraki, 305 Japan

4To whom all correspondence should be addressed.

Xyloglucan oligosaccharides were isolated with various degrees of polymerization (DP) and reduced with tritiated sodium borohydride. The 3H-oligosaccharides were tested for their ability to bind to amorphous and microcrystalline celluloses and to cellulose filter paper. The time course of binding indicated that the radiolabeled oligosaccharides continued to be bound for at least 1 h after heating at 120°C. The binding probably required the organization of the oligosaccharides and celluloses by gradual annealing after heating. Although neither pentasaccharide (glucose: xylose, 3 : 2), heptasaccharide (glucose: xylose, 4 : 3) and nonasaccharide (glucose : xylose : galactose : fucose, 4 : 3 : 1 : 1) failed to bind to the celluloses, binding occurred with oligosaccharides with DP equivalent to more than four consecutive 1,4-ß-glucosyl residues. The extent of binding to the celluloses increased gradually from octasaccharide (glucose: xylose, 5 : 3) to hendecosanosaccharide (glucose/xylose, 12 : 9), with the increase in the DP of 1,4-ß-glucosyl residues. The binding of reduced cello-dextrins to cellulose required at least 4 consecutive 1,4-ß-glucosyl residues. The extent of binding of cellopentitol or cellohexitol to cellulose was similar to that of hendecosanosaccharide, showing lower binding for xyloglucan oligosaccharides in spite of longer chains of 1,4-ß-glucosyl residues. These findings suggest that the mode of binding to cellulose of xyloglucan oligosaccharides is different from that of cello-oligosaccharides.

(Received February 18, 1994; Accepted June 1, 1994)
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