Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Boichenko, V.A.
Right arrow Articles by Schmid, G.H.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Boichenko, V.A.
Right arrow Articles by Schmid, G.H.
Agricola
Right arrow Articles by Boichenko, V.A.
Right arrow Articles by Schmid, G.H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 1994, Vol. 35, No. 5 845-848
© 1994

Short communication

Evidence for Predominant Participation of Photosystem IIß-Units in Nitrogen Formation by Hydroxylamine-Treated Tobacco Chloroplasts

V.A. Boichenko1, K.P. Bader2, V.V. Klimov1 and G.H. Schmid2

1Institute of Soil Science and Photosynthesis, Russian Academy of Sciences 142292 Pushchino, Russia
2Universität Bielefeld, Fakultät für Biologie, Lehrstuhl Zellphysiologie D-33501 Bielefeld 1, Germany

Relative optical cross sections for flash-induced O2- and N2-production with water and 1 mM NH2OH as electron donors to PSII, respectively, as well as that for PSI-mediated O2-uptake have been measured in tobacco chloroplasts by mass spectrometry. In the wild type tobacco these three reactions are driven by three populations of photosynthetic units different with respect to the antenna size. The antenna size of O2-evolving units is twice as large compared to the N2-evolving one, but both of these have the same spectral characteristics in the far red region. In contrast to the wild type, the antenna sizes of O2- and N2-evolving units in the chlorophyll b-deficient tobacco mutant Su/su var. Aurea are the same as the sizes of the N2-evolying units in the wild type chloroplasts. Taking into account the data of Thielen and Van Gorkom [Biochim. Biophys. Acta (1981) 635:111] on a strong difference in the relative amounts of PSIIß and PSIIß in the mutant compared with the wild type (the ratio about 1 : 3 and 3 : 1 , respectively) it is concluded that mainly the stroma-exposed PSIIß units are competent to N2-evolution.

(Received November 11, 1993; Accepted April 25, 1994)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.