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Plant and Cell Physiology, 1994, Vol. 35, No. 5 773-778
© 1994

The Promoter of a Pine Photosynthetic Gene Allows Expression of a ß-Glucuronidase Reporter Gene in Transgenic Rice Plants in a Light-Independent but Tissue-Specific Manner

Naoki Yamamoto1,2, Yuichi Tada3 and Tatsuhito Fujimura3

1National Institute of Agrobiological Resources Tsukuba, Ibaraki, 305 Japan
3Plant Biotechnology Department, Life Science Institute, Mitsui Toatsu Chemicals Inc. Mobara, Chiba, 297 Japan

2To whom correspondence should be addressed.

In angiosperms, the expression of the cab gene that encodes the chlorophyll a/b-binding protein of PSII is light-regulated. However, the pine cab gene is expressed in a light-independent but cell-type-specific manner. In the present study, the cab-6 promoter (1.7 kbp) from pine was fused to a -glucuronidase (GUS) reporter gene and the chimeric gene was introduced into rice protoplasts by electroporation. The GUS expression was studied in the resultant transgenic rice plants. Expression of GUS at a substantial level was confirmed in primary leaves of dark-germinated rice seedlings, and no obvious effect of light on the GUS activity was observed. The expression of GUS was restricted to photosynthetic tissues. The pine cab-6 promoter is, thus, sufficient for induction of light-independent but cell-type-specific expression in cells of a monocot, as is the case in the original pine cells.

(Received December 17, 1993; Accepted April 22, 1994)
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