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Plant and Cell Physiology, 1994, Vol. 35, No. 5 737-741
© 1994

Isolation and Characterization of the Plasma Membrane by Two-Phase Partitioning from the Alkalophilic Cyanobacterium Spirulina platensis

Chang Hong Xu1, Ali Nejidat2, Shimshon Belkin2 and Sammy Boussiba1,3

1Microalgal Biotechnology Laboratory, The Jacob Blaustein Institute for Desert Research, Ben-Gurion University of the Negev Sede-Boker Campus, 84993, Israel
2Environmental Microbiology Laboratory, The Jacob Blaustein Institute for Desert Research, Ben-Gurion University of the Negev Sede-Boker Campus, 84990, Israel

3Corresponding author.

Partition in aqueous dextran-polyethylene glycol two-phase system was used to isolate the plasma membranes from the alkalophilic cyanobacterium Spirulina platensis. The upper phase contained a colorless membranes obtained in relatively short time, 3–4 h. This fraction had a different protein profile than that of the thylakoid fraction obtained in the lower phase. It did not contain cytochrome c-oxidase activity, but retained characteristic Mg2+-ATPase activity that is sensitive to vanadate, stimulated by K+, and has a pH optimum near 8.5. These data support our assumption that the upper phase of the gradient consist of the plasma membrane of S. platensis.

(Received November 25, 1993; Accepted April 12, 1994)
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