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Plant and Cell Physiology, 1993, Vol. 34, No. 7 973-983
© 1993

Elevated Levels of Tubulin Transcripts Accompany the GA3-Induced Elongation of Oat Internode Segments

Nandini Mendu1 and Carolyn D. Silflow

Department of Genetics & Cell Biology and Plant Molecular Genetics Institiute, University of Minnesota St. Paul, MN 55108, U.S.A.

Plant cell elongation involves the deposition of nascent cellulose microfibrils, the orientation of which is regulated in part by the cortical microtubule array. To determine whether changes in tubulin gene expression are associated with cell elongation, we examined GA3-elicited growth of excised internode segments of Avena sativa. Internode elongation was barely detectable after 6 h of GA3 treatment, when levels of beta-tubulin transcripts had increased 5–6 fold over levels found in untreated controls. Elongation continued for 48 h, whereas tubulin transcript levels reached a peak at 24 h and then declined. The extent of internode elongation and the level of tubulin transcript accumulation were dependent upon both concentration and duration of GA3 treatment. Although a 1 h pulse of GA3 treatment was sufficient to induce elongation and enhance tubulin transcript levels, constant GA3 application was necessary to elicit the maximum growth and transcript accumulation responses. Inhibition of internode elongation by abscisic acid and 2,6,dichlorobenzonitrile (an inhibitor of cellulose biosynthesis) or cycloheximide inhibited the GA3-mediated growth response and the accompanying elevation of tubulin transcript levels. The strong correlation we observed between internode elongation and tubulin transcript level suggests that tubulin gene expression is regulated in close association with cell elongation.

1Present address: Deparment of Genetics, North Carolina State University, Raleigh, NC 27695-7614, U.S.A.

(Received January 27, 1993; Accepted July 4, 1993)
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