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Plant and Cell Physiology, 1993, Vol. 34, No. 7 1159-1162
© 1993

Short Communication

N-Ethylmaleimide Blocks the H+ Pump in the Plasma Membrane of Chara corallina Internodal Cells

Izuo Tsutsui1 and Taka-aki Ohkawa2

1Department of Cell Physiology, National Institute for Physiological Sciences Myodaiji, Okazaki, 444 Japan
2Department of Biology, College of General Education, Osaka University Toyonaka 560, Osaka Japan

The sulfhydryl (SH) modifying reagent N-ethylmaleimide (NEM) was applied to the internodal cells of Chara corallina to study the role of SH residues in the activity of the plasma membrane H+ pump. NEM (1 µM) caused a marked depolarizing shift of the resting potential by 64±10mV (n=7) together with depression of the conductance peak at around —200 mV, indicating a marked depression of the H+ pump activity. This effect of NEM was partly reversible, the membrane repolarized and the conductance peak was restored after extracellular washing. The H+ pump inhibitor, dicyclohexylcarbodiimide (DCCD), caused no additive membrane depolarization and/or depression of the H+ pump conductance, in the presence of NEM. This suggests that NEM blocks the H+ pump and that SH residues play a pivotal role in maintaining the H+ pump activity in Chara corallina.

(Received April 10, 1993; Accepted July 29, 1993)
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