Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Cheng, I-P.
Right arrow Articles by Poulton, J. E.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Cheng, I-P.
Right arrow Articles by Poulton, J. E.
Agricola
Right arrow Articles by Cheng, I-P.
Right arrow Articles by Poulton, J. E.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 1993, Vol. 34, No. 7 1139-1143
© 1993

Short Communication

Cloning of cDNA of Prunus serotina (R)-(+)-Mandelonitrile Lyase and Identification of a Putative FAD-Binding Site

I-Ping Cheng1 and Jonathan E. Poulton2

Department of Biological Sciences, The University of Iowa Iowa City, Iowa 52242, U.S.A.

2To whom correspondence should be addressed.

A full-length cDNA clone encoding the flavoprotein (R)-(+)-mandelonitrile lyase was isolated from a black cherry (Prunus serotina) cDNA expression library and sequenced. A putative FAD-binding site was identified near the N-terminus of this enzyme by comparing its deduced amino acid sequence with those of other FAD- and NAD-binding proteins.

1Present address: Department of Pharmacology, University of Medicine & Dentistry of New Jersey, Piscataway, New Jersey 08854, U.S.A.

(Received April 15, 1993; Accepted June 4, 1993)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
K. Gruber, G. Gartler, B. Krammer, H. Schwab, and C. Kratky
Reaction Mechanism of Hydroxynitrile Lyases of the {alpha}/{beta}-Hydrolase Superfamily: THE THREE-DIMENSIONAL STRUCTURE OF THE TRANSIENT ENZYME-SUBSTRATE COMPLEX CERTIFIES THE CRUCIAL ROLE OF LYS236
J. Biol. Chem., May 7, 2004; 279(19): 20501 - 20510.
[Abstract] [Full Text] [PDF]

Home page
 Protein Sci.Home page
I. Dreveny, C. Kratky, and K. Gruber
The active site of hydroxynitrile lyase from Prunus amygdalus: Modeling studies provide new insights into the mechanism of cyanogenesis
Protein Sci., February 1, 2002; 11(2): 292 - 300.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
Z. Hu and J. E. Poulton
Molecular Analysis of (R)-(+)-Mandelonitrile Lyase Microheterogeneity in Black Cherry
Plant Physiology, April 1, 1999; 119(4): 1535 - 1546.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
K. Trummler and H. Wajant
Molecular Cloning of Acetone Cyanohydrin Lyase from Flax (Linum usitatissimum). DEFINITION OF A NOVEL CLASS OF HYDROXYNITRILE LYASES
J. Biol. Chem., February 21, 1997; 272(8): 4770 - 4774.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Wajant and K. Pfizenmaier
Identification of Potential Active-site Residues in the Hydroxynitrile Lyase from Manihot esculenta by Site-directed Mutagenesis
J. Biol. Chem., October 18, 1996; 271(42): 25830 - 25834.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Hasslacher, M. Schall, M. Hayn, H. Griengl, S. D. Kohlwein, and H. Schwab
Molecular Cloning of the Full-length cDNA of (S)-Hydroxynitrile Lyase from Hevea brasiliensis
J. Biol. Chem., March 8, 1996; 271(10): 5884 - 5891.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.