Plant and Cell Physiology, 1993, Vol. 34, No. 7 1107-1115
© 1993
Effects of in Vivo Treatment with Abscisic Acid and/or Cytokinin on Activities of Vacuolar H+ Pumps of Tonoplast-Enriched Membrane Vesicles Prepared from Barley Roots
1Research Institute for Bioresources, Okayama University Kurashiki, Okayama, 710 Japan
2Institute of Low Temperature Science, Hokkaido University Sapporo, 060 Japan
We investigated the effects of in vivo treatment (1 day) of barley roots with abscisic acid (ABA) and/or a cytokinin (6-benzyladenine; BA) on the ATP- and PPi-dependent H+ transport activities of tonoplast-enriched membrane vesicles prepared from the roots. Treatment with ABA significantly increased the two H+ transport activities. By contrast, treatment with BA significantly decreased PPi-dependent H+ transport activity, while the change in ATP-dependent H+ transport activity was small. Increases in the two H+ transport activities caused by treatment with ABA were suppressed during treatment with ABA and BA. Changes in the NO–-inhibitable ATPase activity and the Na+-inhibitable PPiase activity of membrane vesicles after treatment of roots with phytohormone(s) (ABA, BA, ABA + BA) were similar to changes in the ATP- and PPi dependent H+ transport activities of the membrane vesicles, respectively. Immunoblot analysis with antibodies raised against the functional catalytic subunits of the vacuolar H+ pumps (H+- ATPase and H+-PPiase) of mung bean revealed that only the level of the functional catalytic subunit of the H+-PPiase of the membrane vesicles was significantly increased by treatment with ABA alone and in combination with BA. These results suggest that treatment with ABA has a stimulatory effect on the activities of the two H+ pumps of the vacuolar membrane of barley roots, with increase in the level of the catalytic subunit of the H+-PPiase, and that treatment with BA has an inhibitory effect on the two H+ pump activities of the vacuolar membrane without changes in the levels of the catalytic subunits of either H+ pump, with the limitation that treatment with BA has an inhibitory effect only when the activity of the H+-ATPase has been increased by treatment with ABA.
3Present address: Department of Biology, Faculty of Science, Hirosaki University, Hirosaki, 036 Japan
(Received May 16, 1993; Accepted August 16, 1993)
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