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Plant and Cell Physiology, 1993, Vol. 34, No. 7 1063-1072
© 1993

Soybean Lipoxygenase L-4, a Component of the 94-kilodalton Storage Protein in Vegetative Tissues: Expression and Accumulation in Leaves Induced by Pod Removal and by Methyl Jasmonate

Tomohiko Kato1, Yumiko Shirano1, Hiroyuki Iwamoto2 and Daisuke Shibata1

1Mitsui Plant Biotechnology Research Institute Tsukuba, Ibaraki, 305 Japan
2Fukuyama University, Faculty of Engineering, Department of Food Science and Technology Fukuyama, Hiroshima, 729-02 Japan

A lipoxygenase L-4 gene was isolated from a soybean genomic library. The amino acid sequence of lipoxygenase L-4 is highly homologous with the partial amino acid sequence of the 94-kDa vegetative storage protein, vsp94, found in paraveinal mesophyll cells in the leaves of depodded soybean plants. No L-4 expression was observed in maturing seeds. The L-4 gene is highly expressed in the vegetative tissues of young seedlings, including cotyledons, hypocotyls, roots and primary leaves. L-4 expression followed the same pattern as lipoxygenase activity in cotyledons peaking 3 to 5 days after germination, and returning to a basal level by 9 days after germination. L-4 gene expression was low in the roots, stems and leaves of 10-week-old plants. Exposure of 4-week-old plants to atmospheric methyl jasmonate increased L-4 mRNA in leaves. Continuous pod removal from 7-week-old plants over a 2 week period resulted in dramatic accumulation of L-4 mRNA in leaves. Accumulation of the L-4 protein and three other lipoxygenase fractions in the leaves of depodded plants was demonstrated by ion exchange chromatography. These results indicate that lipoxygenase L-4 is a component of vsp94.

(Received May 31, 1993; Accepted August 9, 1993)
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