Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Nakamura, S.
Right arrow Articles by Hayashi, T.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Nakamura, S.
Right arrow Articles by Hayashi, T.
Agricola
Right arrow Articles by Nakamura, S.
Right arrow Articles by Hayashi, T.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 1993, Vol. 34, No. 7 1009-1013
© 1993

Purification and Properties of an Extracellular Endo-1,4-rß-Glucanase from Suspension-Cultured Poplar Cells

Shingo Nakamura and Takahisa Hayashi1

Wood Research Institute, Kyoto University Uji, Kyoto, 611 Japan

1To whom correspondence should be addressed.

An endo-1,4-rß-glucanase (EC 3.2.1.4 [EC] ) was purified to apparent homogeneity from the culture medium of poplar (Populus alba L.) cells by sequential anion-exchange, hydrophobic, and gel-filtration chromatography. The preparation of extracellular rß-glucanase was homogeneous on SDS-polyacrylamide gel electrophoresis (PAGE) and native PAGE. The molecular weight, as determined by SDS-PAGE was 50,000, whereas that determined by gel filtration was 40,000. The isoelectric point (pI) was 5.5. The purified enzyme catalyzed the endohydrolysis of carboxy-methylcellulose with a pH optimum of 6.0 and a km of 1.0 mg ml–1. The enzyme specifically cleaved the 1,4-rß-glucosyl linkages of carboxymethylcellulose, swollen cellulose, lichenan and xyloglucan, although the last was hydrolyzed more slowly than the other tested substrates. The activity of the endo-1,4-rß-glucanase increased up to the early stage of the mid-logarithmic phase of growth and then decreased rapidly, suggesting that the rß-glucanase is induced before cell development.

(Received April 28, 1993; Accepted July 19, 1993)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
S. Hartati, E. Sudarmonowati, Y. W. Park, T. Kaku, R. Kaida, K. Baba, and T. Hayashi
Overexpression of Poplar Cellulase Accelerates Growth and Disturbs the Closing Movements of Leaves in Sengon
Plant Physiology, June 1, 2008; 147(2): 552 - 561.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
R. Tominaga, M. Samejima, F. Sakai, and T. Hayashi
Occurrence of Cello-Oligosaccharides in the Apoplast of Auxin-Treated Pea Stems
Plant Physiology, January 1, 1999; 119(1): 249 - 254.
[Abstract] [Full Text]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.