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Plant and Cell Physiology, 1992, Vol. 33, No. 4 497-501
© 1992

Short Communication

Assembly of Microtubules in a Cytoplasmic Extract of Tobacco BY-2 Miniprotoplasts in the Absence of Microtubule-Stabilizing Agents

Jiang Chang-Jie, Seiji Sonobe and Hiroh Shibaoka

Department of Biology, Faculty of Science, Osaka University Toyonaka, Osaka, 560 Japan

The assembly of microtubules occurred even in the absence of microtubule-stabilizing agents in a cytoplasmic extract obtained from evacuolated protoplasts (miniprotoplasts) of tobacco BY- 2 cells. The assembled microtubules were arranged side by side forming big bundles and two types of bridge-like structure were observed between adjacent microtubules.

(Received October 31, 1991; Accepted March 20, 1992)
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