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Plant and Cell Physiology, 1992, Vol. 33, No. 4 395-406
© 1992

Radiolabelling Studies of Lipids in the Marine Cryptomonad Chroomonas salina in Relation to Fatty Acid Desaturation

R. James Henderson and Elizabeth E. Mackinlay

N.E.R.C. Unit of Aquatic Biochemistry, Department of Biological and Molecular Science, University of Stirling Stirling FK9 4LA, Scotland, U.K.

Cells of Chroomonas salina were exposed to [14C]acetate, [l4C]16:0, [14C]18:0, [14C]18:1(n-9), [14C]18:2(n–6) or [14C]18:3(n–3) for 1 h and then incubated for 24 h in non-radioactive medium. At the end of the pulse period, non-glycolipid polar lipids contained the highest proportions of radioactivity incorporated from [14C]acetate and [14C]18:3(n–3) whereas with [14C]16:0, [14C]18:1 and [14C]18:2(n–6), triacylglycerols were most highly labelled. 14C-18:0 was recovered mainly as non-esterified fatty acid. Monogalactosyldiacylglycerol initially contained 17% of label incorporated from [14C]acetate but less than 3% of that from [14C]fatty acids. With all substrates, excluding [14C]18:0, a gradual transfer of label from polar lipids to triacylglycerols was observed during the chase period. Saturates and monoenes synthesised from [14C]acetate were mostly transfered from phospholipids and glycolipids to neutral lipid without further desaturation. Most of the incorporated 14C-fatty acids remained unchanged and only with [14C]18:3(n–3) was substantial amounts of label recovered in penta- and hexaenoic fatty acids. The results indicate that, under the conditions of the study, lipid synthesis in the algae was heavily dominated by triacylglycerol formation and that the mechanisms of fatty acid desaturation in this species may differ from those in higher plants.

(Received December 10, 1991; Accepted March 6, 1992)
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