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Plant and Cell Physiology, 1992, Vol. 33, No. 2 171-176
© 1992

Article

Characteristics of Aluminum-Phosphate-Adapted Carrot Cells: Uptake and Utilization of the Phosphate

Hiroyuki Koyama1,4, Kunihiko Ojima2 and Tomoyuki Yamaya3

1Laboratory of Plant Cell Technology, Department of Biotechnology, Faculty of Agriculture Yanagido 1-1, Gifu, 501-11 Japan
2Laboratory of Plant Nutrition, Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University 1-1 Tsutsumidori-Amamiyamachi, Aoba-Ku, Sendai, 981 Japan
3Laboratory of Plant Cell Engineering, Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University 1-1 Tsutsumidori-Amamiyamachi, Aoba-Ku, Sendai, 981 Japan

4To whom correspondence should be addressed.

To elucidate the mechanism responsible for the superior growth of a selected line of carrot cells (Daucus carota L. cv MS Yonsun) in medium that contained AIPO4, kinetic studies of the uptake of phosphate and the efficiency of utilization of phosphate were performed with the selected cells and the wild-type cells. When the two cell lines were grown in a medium with adequate soluble phosphate (2 mM), there was no difference between their growth rates. Rates of increase in fresh weight as a function of increasing concentration of phosphate in the medium were also identical between the cell lines, indicating that the efficiency of utilization of phosphate by the selected cell line was similar to that by the wild-type cells. However, rate of uptake of phosphate by the selected cells under phosphate limited conditions (20µM NaH2PO4 at pH 5.6) was about 5-fold higher than that by the wild-type cells. Apparent Km values for the uptake of phosphate were calculated to be 13.6 and 9.1 µM for the selected and the wild-type cells, respectively. The Vmax valuewas estimated to be 88.8 nmol per g fresh weight per min for the selected cells and 28.2 for thewild-type cells. Thus, the selected cells has an enhanced system for uptake of phosphate wherebythere was an increase in the rate of the uptake without any dramatic change in the affinity for phosphateions.

(Received September 21, 1991; Accepted December 25, 1991)
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