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Plant and Cell Physiology, 1992, Vol. 33, No. 2 139-149
© 1992

Stimulation of the Extrusion of Protons and H+-ATPase Activities with the Decline in Pyrophosphatase Activity of the Tonoplast in Intact Mung Bean Roots under High-NaCl Stress and Its Relation to External Levels of Ca2+ Ions

Yoshiyuki Nakamura1, Kunihiro Kasamo2, Makato Sakata3 and Eiji Ohta1

1Department of Instrumentation Engineering, Faculty of Science and Technology,Keio University Yokohama, 223 Japan
2Molecular Function Laboratory, National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries Kannondai, Tsukuba, Ibaraki, 305 Japan
3School of Medicine, Kyorin University Mitaka, Tokyo, 181 Japan

Extrusion of protons as a response to high-NaCl stress in intact mung bean roots was investigated at different external concentrations of Ca2+ ions ([Ca2+]ex). The extrusion of protons was gradually enhanced in the roots exposed to 100 mM NaCl, and high [Ca2+]ex diminished this enhancement of the extrusion. Vesicles of plasmalemma and tonoplast were prepared from the roots and the H+-translocating ATPase (H+-ATPase) activities associated with the two types of membrane and the H+-pyrophosphatase (H+-PPase) activity of the tonoplast were assayed. The plasmalemma ATPase was stimulated in parallel with dramatic increases in the intracellular concentration of Na+([Na+]in). High [Ca2+]ex prevented the increase in [Na+]in and diminished the stimulation of ATPase activity. The tonoplast ATPase showed a rapid response to salt stress and was similarly stimulated even at high [Ca2+]M. The activities of both ATPases were, however, insensitive to concentrations of Na+ ions up to 100 HIM. By contrast, H+-PPase activity of the tonoplast was severely inhibited with increasing [Na+]in under salt stress and recovered with high [Ca2+]ex. These findings suggest that high-NaCl stress increases the intracellular concentration of Na+ ions in mung bean roots, which inhibits the tonoplast H+-PPase, and the activity of the plasmalemma H+-ATPase is thereby stimulated and regulates the cytoplasmic pH.

(Received March 26, 1991; Accepted December 13, 1991)
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