Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Kawasaki, S.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Kawasaki, S.
Agricola
Right arrow Articles by Kawasaki, S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 1991, Vol. 32, No. 5 721-728
© 1991

Article

Extensin Secreted into the Culture Medium of Tobacco Cells II. Extensin Subfamilies of Similar Composition

Shinji Kawasaki

National Institute of Agrobiological Resources, Tsukuba Science City Ibaraki, 305 Japan

Combining acetic acid extraction and high-performance gel chromatography in guanidine HCl, extensin secreted into the medium by tobacco (Nicotiana tabacum L. var Xanthi) culture cells was separated into three component molecules, namely a major 74-kDa, and two minor 45-and 28-kDa components, in addition to larger oligomers. The sizes of these native extensin molecules were first reasonably assessed using this gel-chromatography system. After deglycosylation with hydrogen fluoride, the separation was improved and the estimated molecular sizes were reduced to 52 kDa, 34 kDa and 18 kDa, respectively. The amino acid compositions of these components were similar, and N-terminal sequences of the 52- and 34-kDa components coincided. The relative abundance of the components was as follows: oligomers, 46%; 52-kDa, 44%; 34-kDa, 7.7%; 18-kDa, 2.2%; respectively, on a protein basis (w/w). Fluorography of the acid extract of microsomes from cells labelled with 14C-proline revealed only one precursor band of 110-kDa or 42-kDa under the glycosylating or non-glycosylating conditions, respectively. The smaller components in the medium may be derived, by proteolytic cleavage, from the major extensin molecule after secretion.

(Received October 15, 1990; Accepted May 15, 1991)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.