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Plant and Cell Physiology, 1991, Vol. 32, No. 5 713-720
© 1991

Article

Enzymic Dissociation of Zea Shoot Cell-Wall Polysaccharides V. Dissociation of Xyloglucan by Urea1

Yoji Kato2 and Donald J. Nevins3

2Laboratory of Food Science, Faculty of Education, Hirosaki University Hirosaki, 036 Japan
3Department of Vegetable Crops, University of California, Davis California 95616 U.S.A.

3Author to whom correspondence should be addressed.

A procedure has been devised to extract and identify structural components of the xyloglucan of Zea mays L. (hybrid B73 × Mo 17) shoot cell-walls. A water-insoluble fraction of Zea shoot cell-walls, after pretreatment with purified Bacillus subtilis (1 -> 3),(1 -> 4)-ß-D-glucan 4-glucanohydrolase, purified B. subtilis endo-(l -> 4)-ß-xylanase and an enzyme preparation from B. subtilis enriched in glucuronoxylanase (Kato and Nevins 1984a, Nishitani and Nevins 1991), was subsequently treated with 7 M urea. The carbohydrates (0.8% of the water-insoluble fraction of Zea shoot cell-walls) liberated by the urea treatment, were comprised of xyloglucan polymers with molecular weights which varied from 1.0 × 104 to 4.0 7times; 104 Da. Other wall fragments associated with the isolated polymer suggest covalent bonding of xyloglucan to other polysaccharides. Structural analyses of the xyloglucan polymers reveal a cellulose-like backbone with about 35% of the C-6 positions substituted with xylose and other sugars. About 80% of xyloglucan present in the enzyme-pretreated water-insoluble fraction of Zea shoot cell-walls was liberated by the urea treatment. The procedure avoids the use of alkali in the solubilization of xyloglucan.

1Supported in part by National Science Foundation research grants PCM 7818588 and DMB 8505901.

(Received September 10, 1990; Accepted May 15, 1991)
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