Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Ishige, F.
Right arrow Articles by Imaseki, H.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Ishige, F.
Right arrow Articles by Imaseki, H.
Agricola
Right arrow Articles by Ishige, F.
Right arrow Articles by Imaseki, H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 1991, Vol. 32, No. 5 681-690
© 1991

Article

The Effects of Ethylene on the Coordinated Synthesis of Multiple Proteins: Accumulation of an Acidic Chitinase and a Basic Glycoprotein Induced by Ethylene in Leaves of Azuki Bean, Vigna angularis

Fumiharu Ishige1, Ken'ichi Yamazaki1, Hitoshi Mori2 and Hidemasa Imaseki1,2,3

1Research Institute for Biochemical Regulation, School of Agricultural Sciences, Nagoya University Nagoya, 464-01 Japan
2National Institute for Basic Biology Okazaki, 444 Japan

3 To whom correspondence should be addressed.

The ethylene-induced synthesis and accumulation of proteins were studied in the primary leaves of azuki bean (Vigna angularis). Seven different proteins, designated AZ17, 23, 27, 32, 35, 36, 42 according to their molecular masses, were synthesized and accumulated in response to ethylene. AZ27 and AZ42 were purified to homogeneity and characterized. AZ27 was identified as an acidic chitinase and accumulated in the extracellular space. The sequence of the 40 N-terminal amino acids of AZ27 showed no similarity to that of a basic chitinase from bean and tobacco, but it was highly homologous to that of a chitinase from virus-infected cucumber leaves. AZ42 was identified as a glycoprotein that accumulated intracellularly. A search for proteins with sequences homologous to an internal sequence of 18 amino acids in AZ42 was unsuccessful. Immunochemical examination revealed that auxin and abscisic acid enhanced the ethylene-induced accumulation of AZ27 but not of AZ42. In contrast, levels of AZ42 were not affected by auxin or abscisic acid, but cytokinin suppressed the accumulation of one of the doublet bands of AZ42. Translatable mRNAs coding for AZ27 and AZ42 were not present in leaves that had not been treated with ethylene, but levels of these mRNAs increased after such treatment.

(Received March 1, 1991; Accepted May 8, 1991)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.