Plant and Cell Physiology, 1991, Vol. 32, No. 5 593-601
© 1991
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A Ca2+- and Voltage-Dependent Cl– -Sensitive Anion Channel in the Chara Plasmalemma: A Patch-Clamp Study
1Department of Biophysical Engineering, Faculty of Engineering Science Osaka University, Toyonaka, 560 Japan
2Department of Biology, College of General Education, Osaka University Toyonaka, 560 Japan
3Department of Cell Physiology, National Institute for Physiological Sciences Okazaki, 444 Japan
The inside-out patch-clamp technique was applied to the plasmolyzed plasmalemma of inter-nodes of Chara corallina without enzymatic treatment. We found two different types of channel activity that were CP–-sensitive. Both types of channel were Ca2+-dependent. However, the one that exhibited greater dependence on Ca2+ ions was the focus of our studies, and we named it the Ca2+-dependent CP–-sensitive anion channel. When the concentration of Ca2+ ions on the cyto-plasmic side was 1.0 µM, the Ca2+-dependent CP–-sensitive channel opened most frequently between approximately –80 and –100 mV. At 10 µM Ca2+, it opened less frequently, and at 0.1 µM Ca2+ it scarcely opened at all. These observations indicate that the anion channel of interest is voltage-dependent over a restricted range of concentrations of Ca2+ ions. The dependence on Ca2+ and voltage of the channel can explain the behavior of the excitable Ca2+-activated Cl– channel in the Chara plasmalemma. The channel activity was blocked by several antagonists of calmodulin.
4 Present Address: Department of Biology, College of General Education, Osaka University, Toyonaka, 560 Osaka, Japan
(Received October 8, 1990; Accepted April 4, 1991)
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