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Plant and Cell Physiology, 1991, Vol. 32, No. 3 409-417
© 1991

Article

Immunofluorescence Microscopical Observation of Cortical Microtubule Arrangement as Affected by Gibberellin in d5 Mutant of Zea mays L.

Kyoko Ishida1 and Masayuki Katsumi

Biology Department, International Christian University Osawa, Mitaka-shi, Tokyo, 181 Japan

The relationship between the dwarf habit of growth and cortical microtubule (MT) orientation as affected by gibberellin was examined using a gibberellin responding dwarf mutant of Zea mays L. (d5). The 4 mm portion of the mesocotyl below the coleoptilar node of dark-grown seedlings was divided into four 1 mm segments. MTs were observed by means of immunofluorescence microscopy on the tangential surface of the epidermal cells (EP-t), the radial surfaces of epidermal (EP-r) and cortex cells (C-r) in both normal and d5 seedlings. MT arrangement in EP-t was transverse, oblique and/or longitudinal. In lower regions, cells with transversely oriented MTs decreased, while those with obliquely/longitudinally oriented MTs increased. The frequency of the occurrence of transversely oriented MTs was much higher in normal than in d5. In EP-r almost all the cells of the four regions had transversely oriented MTs. In d5 seedlings only a few percent of cells had obliquely oriented MTs. In C-r all the cells of the four regions of normal seedlings had transversely oriented MTs, while in d5, 20% of the uppermost region cells showed oblique/longitudinal orientation and more in lower regions. By GA3 (100µM) treatment for 24 h, the growth of d5 mesocotyls was stimulated, and the orientation of MTs in EP-t, EP-r and C-r of any regions became transverse.

1Present address: Department of Forestry, Faculty of Agriculture, the University of Tokyo, Yayoi-1-Chome, Bunkyo-ku, Tokyo, 113 Japan

(Received August 16, 1990; Accepted January 22, 1991)
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