Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Minocha, S. C.
Right arrow Articles by Samuelsen, A. I.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Minocha, S. C.
Right arrow Articles by Samuelsen, A. I.
Agricola
Right arrow Articles by Minocha, S. C.
Right arrow Articles by Samuelsen, A. I.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 1991, Vol. 32, No. 3 395-402
© 1991

Article

Polyamines and Somatic Embryogenesis in Carrot. III. Effects of Methylglyoxal bis(guanylhydrazone)l

Subhash C. Minocha, Nancy S. Papa2, A. Jamal Khan3 and Andrew I. Samuelsen

Department of Plant Biology, University of New Hampshire Durham, N.H., 03824 U.S.A.

The effects of methylglyoxal bis(guanylhydrazone) (MGBG) on somatic embryogenesis, titres of cellular polyamines and 1-aminocyclopropane-l-carboxylic acid, and the activity of arginine decarboxylase (ADC) were studied using suspension cultures of carrot (Daucus carota L.). Whereas MGBG (0.1–0.5 mM) significantly inhibited the cellular levels of spermidine and spermine, putrescine levels were higher in the treated tissue. MGBG also promoted ACC levels in the cells. The activity of ADC was inhibited in the presence of MGBG. Somatic embryogenesis in the auxin-free medium was completely inhibited by MGBG. The effects of MGBG on somatic embryogenesis were reversed by 0.1–0.2 mM spermidine but not by spermine. These results are consistent with the suggestion that an interaction between ethylene and polyamine bio-synthetic pathways through competition for the common precursor, S-adenosylmethionine, plays an important role in the development of somatic embryos in carrot cell cultures.

1Scientific Contribution Number 1649 from the New Hampshire Agricultural Experiment Station. This research was supported by NSF Grant No. DCB-8615945, Central University Research Funds, UNH, and NHAES Project H-233

2Present address: Nancy S. Papa, In Vitron Corporation, 4649 LeBourget Drive, St. Louis, MO 63134, U.S.A.

3Present address: Dr. A. Jamal Khan, Department of Plant Science, College of Agriculture, Sultan Qaboos University, P.O. Box-32484, Al-Khod, Muscat, Sultanate of Oman

(Received September 17, 1990; Accepted January 21, 1991)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.