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Plant and Cell Physiology, 1991, Vol. 32, No. 3 379-384
© 1991

Article

Factors Controlling Induction of External Carbonic Anhydrase and Change in K½(CO2) of Photosynthesis in Chlorella regularis

Yasuyuki Umino, Akira Satoh and Yoshihiro Shiraiwa1

Department of Biology, Faculty of Science, Niigata University Ikarashi 2-8050, Niigata, 950-21 Japan

1To whom correspondence should be addressed.

Transfer of algal cells of Chlorella regularis from 3% CO2 in air into ordinary air in the light increased external carbonic anhydrase (CA) activity as well as photosynthetic affinity for CO2 by several-fold within 2 h. Since no noticeable difference was observed in CA activity between intact cells and cell homogenates, CA seemed to be mainly localized on the cell surface. Changes in CA activity and K½(CO2) of photosynthesis were not observed in the dark. CA induction was 50%-inhibited by incubation with 10 µM DCMU during adaptation of high-CO2 cells to air, whereas it was considerably suppressed when high-CO2 cells preincubated with DCMU in the light for 6 h or without DCMU in the dark for 24 h were used. The change in K½(CO2) of photosynthesis was only slightly affected by DCMU. Uncoupler like carbonylcyanide-m-chlorophenyl-hydrazone (CCCP) and inhibitors of mitochondrial respiration (KCN plus salicylhydroxamic acid) suppressed CA induction during adaptation of high-CO2 cells to low CO2 conditions.

These results suggest that photosynthesis is not essential for CA induction in Chlorella regularis when some amounts of photosynthetic products are previously stored in the cells and respiration is active. A decrease in K½(CO2) of photosynthesis during adaptation from high to low CO2 was mostly independent on photosynthesis. However, light is essential for both phenomena.

(Received July 16, 1990; Accepted January 21, 1991)
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