Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Oyama, H.
Right arrow Articles by Wada, M.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Oyama, H.
Right arrow Articles by Wada, M.
Agricola
Right arrow Articles by Oyama, H.
Right arrow Articles by Wada, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 1990, Vol. 31, No. 8 1229-1238
© 1990

Article

Phytochrome in the Fern, Adiantum capillus-veneris L.: Spectrophotometric Detection in Vivo and Partial Purification

Haruo Oyama1, Kotaro T. Yamamoto2 and Masamitsu Wada1

1Department of Biology, Faculty of Science, Tokyo Metropolitan University 2-1-1 Fukazawa, Setagaya-ku, Tokyo, 158 Japan
2Division of Biological Regulation, National Institute for Basic Biology Myodaijicho, Okazaki, Aichi, 444 Japan

Spectrophotometric studies of fern phytochrome were performed using dark-grown leaves of Adiantum. The absorbance difference spectrum between the red- and far-red-light irradiated sample showed a photoreversible absorbance change in the far-red region, with a maximum located at 728–730 nm. The concentration of phytochrome was highest at the leaf tips and decreased gradually along the leaf axis. As in the case of angiosperm phytochrome, the level of fern phytochrome decreased under continuous white light, and the level increased again when deetiolated tissue was transferred back to the dark. When the fern tissue was exposed to a pulse of red light, the dark reversion of PFR to PR took place with almost no destruction of PFR. Phytochrome could be extracted from light-grown young leaves of the fern with a slightly alkaline, aqueous buffer that contained 1 M NaCl. The difference spectrum of the partially purified phytochrome from fern was similar to that of partially degraded phytochrome from angio-sperms. A polyclonal antibody raised against phytochrome from etiolated rye seedlings immuno-stained (albeit weakly) a 110-kDa polypeptide after fractionation by SDS-polyacrylamide gel electrophoresis of the preparation of fern phytochrome. The band was very probably fern phytochrome since it emitted zinc-induced fluorescence.

(Received July 12, 1990; Accepted October 5, 1990)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
H. Hanzawa, T. Shinomura, K. Inomata, T. Kakiuchi, H. Kinoshita, K. Wada, and M. Furuya
Structural requirement of bilin chromophore for the photosensory specificity of phytochromes A and B
PNAS, April 2, 2002; 99(7): 4725 - 4729.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
T. Murata and M. Sugai
Photoregulation of Asymmetric Cell Division Followed by Rhizoid Development in the Fern Ceratopteris Prothalli
Plant Cell Physiol., December 1, 2000; 41(12): 1313 - 1320.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S.-H. Wu, M. T. McDowell, and J. C. Lagarias
Phycocyanobilin Is the Natural Precursor of the Phytochrome Chromophore in the Green Alga Mesotaenium caldariorum
J. Biol. Chem., October 10, 1997; 272(41): 25700 - 25705.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.