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Plant and Cell Physiology, 1990, Vol. 31, No. 8 1183-1190
© 1990

Article

Effects of a Plant-Growth Regulator, Prohexadione, on the Biosynthesis of Gibberellins in Cell-Free Systems Derived from Immature Seeds

Ishizue Nakayama1, Yuji Kamiya2, Masatomo Kobayashi2, Hiroshi Abe1 and Akira Sakurai2

1Life Science Research Institute, Kumiai Chemical Industry Co., Ltd. Kikugawa-cho, Ogasa-gun, Shizuoka, 439 Japan
2The Institute of Physical and Chemical Research Wako-shi, Saitama, 351-01 Japan

Inhibition of the biosynthesis of gibberellins by prohexadione, 3,5-dioxo-4-propionylcyclo-hexanecarboxylic acid, was studied with cell-free systems derived from immature seeds of Cucur-bita maxima, Phaseolus vulgaris and Pisum sativum. Prohexadione, at a concentration of 10–4 M, inhibited C-7 oxidation of GA12-aldehyde, C-20 oxidation of GA15, conversion of C20-gib-berellins to C19-gibberellins, 3ß-hydroxylation, 2,3-dehydrogenation of GA20, 2,3-epoxidation of GA5 and 2ß-hydroxylation of GA9 and GA20. The 3ß-hydroxylase activity appeared to be more sensitive to prohexadione than were the C-20 oxygenase and the 2ß-hydroxylase activities. The conversion of mevalonic acid to GA12-aldehyde and the 13-hydroxylation of GA12 were not affected by prohexadione at a concentration of 3 × 10–4 M. All of the steps inhibited by prohexadione are oxidation steps catalyzed by soluble enzymes that require 2-oxoglutarate, Fe2+ and oxygen, and all of them occur distal to the synthesis of GA12-aldehyde in the biosynthesis of gibberellins.

(Received April 4, 1990; Accepted September 14, 1990)
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