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Plant and Cell Physiology, 1990, Vol. 31, No. 8 1169-1176
© 1990

Article

Changes to Proteins in the Shoot Meristem of Silene coeli-rosa during the Transition to Flowering

M. Taylor1, D. Francis1, J. Rembur2 and A. Nougarède2

1School of Pure and Applied Biology, University of Wales P.O. Box 915, Cardiff CF1 3TL U.K.
2Laboratoire de Cytologie experimental et morphogenèse végétale, Université Pierre et Marie Curie Bâtiment N2, 4, place Jussieu, 75252 Paris cedex 05, France

Changes in proteins were measured to test whether they were linked to flowering, the cell cycle or both in Silene coeli-rosa shoot apices. Seeds were germinated and grown at 20°C in short days (SD) of 8 h light from fluorescent and tungsten (F + T)/16h darkness for 28 days (day 0). Plants were then exposed to: 7 long days (LD)+2 SD (inductive), 7 LD + 48 h darkness (inductive) or 7 dark-interrupted (di) LD + 2SD (non-inductive), where each LD and diLD comprised 8 h F + T/16 h T, 8 h F+T/l h darkness/15 h T, respectively. There were no qualitative differences in polypeptide composition in the LD and SD treatments on days 0, 5, 6 and 7 but 60 new polypeptides were detected on LD8 and some modifications in the intensity of common spots also occurred. These qualitative and quantitative changes were not altered following 7 LD + 48 h darkness, which is known to suppress synchronisation of the cell cycle but not flowering. Thus, changes on day 8 are linked to flowering. Transient increases in protein per unit area were detected in prophase cells on days 0, 3, 4 while sustained increases occurred on days 7 and 8. The changes on days O, 3 and 4 were suppressed by the diLD treatment. Thus, quantitative changes to proteins, co-inciding with known LD-induced changes to the cell cycle, are linked to flowering.

(Received April 2, 1990; Accepted September 12, 1990)
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