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Plant and Cell Physiology, 1990, Vol. 31, No. 8 1079-1082
© 1990

Article

Variation and Estimation of the Differential Absorption Coefficient of P-700 in Spinach Photosystem I Preparations

Kintake Sonoike1,2 and Sakae Katoh2

1Solar Energy Research Group, The Institute of Physical and Chemical Research (RIKEN) Wako, Saitama, 351-01 Japan
2Department of Biology, Faculty of Sciences, University of Tokyo Hongo, Bunkyo-ku, Tokyo, 113 Japan

Treatment of spinach Photosystem I particles with detergents induced an apparent blue shift of chlorophyll {alpha} at 690 nm in the difference spectrum of P-700. As a consequence of the band shift, the differential absorption coefficient of P-700 was increased from 63 to 87 mM–1 .cm–1. A curve was presented, with which changes in the apparent differential absorption coefficient of P-700 can be estimated by measuring magnitudes of light-induced absorption decreases at 680 nm and 700 nm. The curve was compared with that for cyanobacterial preparations and the mechanism of the detergent-induced band shift was discussed.

(Received July 11, 1990; Accepted August 23, 1990)
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