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Plant and Cell Physiology, 1990, Vol. 31, No. 8 1071-1077
© 1990

Article

Response of Glutamine Synthetase and Glutamate Synthase Isoforms to Nitrogen Sources in Rice Cell Cultures

Toshihiko Hayakawa1, Kazunari Kamachi1, Mizushiro Oikawa1, Kunihiko Ojima1 and Tomoyuki Yamaya2,3

1Laboratory of Plant Nutrition, Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University 1-1 Tsutsumidori-Amamiyamachi, Aoba-Ku, Sendai, 981 Japan
2Laboratory of Plant Cell Engineering, Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University 1-1 Tsutsumidori-Amamiyamachi, Aoba-Ku, Sendai, 981 Japan

3To whom correspondence should be addressed

As a model system with no photorespiration and no long distance transport, rice cell cultures (Oryza saliva L. cv Sasanishiki) were used to investigate the effect of nitrogen sources on the levels of isoforms of glutamine synthetase (GS) and glutamate synthase (GOGAT). Isoforms of GS and GOGAT were analyzed by immunoblotting methods and their activities in early growth phase of the cells. Cytosolic type GS (41 kDa subunit) and NADH-GOGAT were the major isoforms in the rice cells grown in normal R-2 medium. However, contents of plastid type GS (44 kDa subunit) and Fd-GOGAT increased in response to NO–3 supply. NADH-GOGAT activity also increased following the supply of NO–3. In vitro translated products from poly(A)+RNA prepared from the cells showed that the precursor of plastid type GS (49 kDa) was detected at 48 h after the inoculation. Supply of NH+4 resulted in an increase in NADH-GOGAT activity but had no effect on the levels of Fd-GOGAT, of polypeptides of the plastid type GS or of the corresponding mRNAs.

(Received May 30, 1990; Accepted August 23, 1990)
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