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Plant and Cell Physiology, 1990, Vol. 31, No. 8 1063-1069
© 1990

Article

Identification and Semi-Quantification of Gibberellins from the Pollen and Anthers of Zea mays by Immunoassay and GC/MS

Isomaro Yamaguchi1, Hideyuki Nakazawa1, Ryusuke Nakagawa1, Yoshihito Suzuki1, Shin Kurogochi2, Noboru Murofushi1, Nobutaka Takahashi1 and Elmar W. Weiler3

1Department of Agricultural Chemistry, The University of Tokyo Bunkyo-ku, Tokyo, 113 Japan
2Nihon Tokushu-Noyaku Co. Ltd. Yuki-Shi, Ibaraki-ken, 307 Japan
3Department of Plant Physiology, Ruhr-University Bochum D 4630 Bochum 1, Federal Republic of Germany

Radioimmunoassays and enzyme-linked immunosorbent assays for methyl esters of gibberellins A1, A3, A4, and A7 were established using an antiserum specific for GA1-Me. The antiserum was characterized by high titer and specificity for such C19-GAs with 3ß-hydroxyl group as GA1, GA3, GA4 and GA7. Combination of this antiserum and HPLC enabled us to identify and quantify GA, and GA4 from the pollen of Zea mays with a high degree of reliability. Similarly, identification and quantification of GA9 and GA20 were also made possible by use of an antiserum specific for GA20-Me. Combined use of immunoassays and GC/MS enabled us to identify nine GAs from the pollen and four from the anthers of Zea mays. The identification of non-13-hydroxylated GAs, such as GA4 and GA9, in addition to 13-hydroxylated GAs from the pollen and the anthers suggests that the early-non-hydroxylation pathway, as well as the early-13-hydrox-ylation pathway, operates in the male reproductive organs of Zea mays, and that the organ-specific biosynthesis and/or localization of GAs in Zea mays is similar to that in Oryza saliva.

(Received May 7, 1990; Accepted August 20, 1990)
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