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Plant and Cell Physiology, 1990, Vol. 31, No. 4 539-544
© 1990

Article

Hydrogen Peroxide-Dependent Oxidation of 3,4-Dihydroxyphenylalanine in Vacuoles of Mesophyll Cells of Vicia faba L. Participation of Peroxidase in the Oxidation

Umeo Takahama and Takeshi Egashira

Department of Biology, Kyushu Dental College Kitakyushu, 803 Japan

Peroxidase activity and 3,4-dihydroxyphenylalanine (DOPA) were found in vacuoles isolated from mesophyll protoplasts of Vicia faba L. A peroxidase isozyme localized in vacuoles migrated to the cathode during electrophoresis at pH 8.7, indicating that the vacuole peroxidase was a basic isozyme. When isolated vacuoles were treated with 2 mM H2O2, dopachrome, a product of oxidation of DOPA, was formed in a reaction that was inhibited by KCN and NaN3. These results suggest that DOPA can serve as a donor of electrons to the peroxidase in vacuoles.

(Received December 25, 1989; Accepted March 22, 1990)
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