Plant and Cell Physiology, 1990, Vol. 31, No. 4 519-526
© 1990
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Classification and Characterization of cDNA That Encodes the Light-Harvesting Chlorophyll a/b Binding Protein of Photosystem II from Rice
National Institute of Agrobiological Resources Kannondai, Tsukuba Science City, Ibaraki, 305 Japan
Thirteen cDNA clones for the light-harvesting chlorophyll a/b binding protein of photosystem II (LHCPII) from rice were constructed; they were grouped into two classes that were based on restriction enzyme maps. The nucleotide sequences from two clones that belonged to different classes were determined and compared. The clones contained a 798-bp and 792-bp open reading frame capable of coding for polypeptides with 265 and 263 amino acids, respectively. These sequences differ from each other, not only in the 5'- and 3'-noncoding regions, but also in the transit peptide regions and the N-terminal regions of the mature polypeptides. By contrast, the internal and C-terminal regions of the mature polypeptides are quite similar. It has been demonstrated that two types of LHCPII, with different structures and designated type I and type II, exist in some plants. A comparison of the deduced amino acid sequences between the rice clones and analogous sequences from other plants indicates that one of the pair of clones corresponds to type I LHCPII and the other to type II. Genomic Southern blot analysis using a specific probe for each type of LHCPII suggested that the genes for both types of protein are present as small multigene families in the rice genome. Northern blot analysis, using the specific probes, revealed that mRNAs for type I and type II LHCPII were barely detectable in dark-grown rice seedlings but they accumulated rapidly and in a similar manner after illumination of seedlings with white light. However, the amount of type I LHCPII mRNA was three times larger than that of type II mRNA in the greening seedlings. The results indicate that the mechanism for the regulation of the expression of the genes by light are similar but the amounts of mRNAs for type I and type II are different.
(Received February 19, 1990; Accepted March 14, 1990)
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